Callies moved to Honolulu, Hawaii with her family when she was one. Throughout her youth, she expressed an interest in acting through participating in various school plays at Punahou School.
While her parents are professors at the University of Hawaii, Manoa, Callies did not follow in their footsteps. Instead, she chose to pursue a career in acting. After graduating from high school, she entered Dartmouth College in Hanover, New Hampshire. In conjunction with her studies, she continued her involvement in theatre.She continued her education at Denver's National Theater Conservatory where she obtained her Master of Fine Arts degree in 2002.
"As hard as we all tried, the Prison Break powers that be and I were unable to find a way to meet both the needs of the story and the needs of my family. We parted wishing each other well. I had a wonderful time working with the creative team and have a world of respect for all of them; they took great care of Dr. Sara. I'm also enormously grateful to the fans. They've been so gracious and supportive, and I hope they continue to enjoy the show."